bpk1520 puror (Addgene inc)
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bpk1520 puror
Bpk1520 Puror, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bpk1520+puror/BPK1520+(Plasmid+%2365777)/pmc12827430-225-22-23
Average 94 stars, based on 103 article reviews
Bpk1520 Puror, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bpk1520+puror/BPK1520+(Plasmid+%2365777)/pmc12827430-225-22-23
Average 94 stars, based on 103 article reviews
bpk1520 puror - by Bioz Stars,
2026-09
94/100 stars
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Polymerase Chain Reaction:Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2 Article Snippet: To generate the CMV-dCas9_puroR vector (Addgene #219825), we first removed the KRAB and MeCP2 inhibition domains from dCas9-KRAB-MeCP2 (Addgene 110821) by inverse PCR. .. We circularized the PCR product using KLD Enzyme Mix (NEB) to generate a new backbone, and we inserted the puromycin resistance cassette which was amplified from Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2. Article Snippet: To generate the CMV-dCas9_puroR vector (Addgene #219825), we first removed the KRAB and MeCP2 inhibition domains from dCas9-KRAB-MeCP2 (Addgene 110821) by inverse PCR. .. We circularized the PCR product using KLD Enzyme Mix (NEB) to generate a new backbone, and we inserted the puromycin resistance cassette which was amplified from Amplification:Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2 Article Snippet: To generate the CMV-dCas9_puroR vector (Addgene #219825), we first removed the KRAB and MeCP2 inhibition domains from dCas9-KRAB-MeCP2 (Addgene 110821) by inverse PCR. .. We circularized the PCR product using KLD Enzyme Mix (NEB) to generate a new backbone, and we inserted the puromycin resistance cassette which was amplified from Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2. Article Snippet: .. To generate pXR003_puroR (Addgene #219819), we amplified the puromycin resistance cassette from Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2. Article Snippet: To generate the CMV-dCas9_puroR vector (Addgene #219825), we first removed the KRAB and MeCP2 inhibition domains from dCas9-KRAB-MeCP2 (Addgene 110821) by inverse PCR. .. We circularized the PCR product using KLD Enzyme Mix (NEB) to generate a new backbone, and we inserted the puromycin resistance cassette which was amplified from Cloning:Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2 Article Snippet: To generate the CMV-dCas9_puroR vector (Addgene #219825), we first removed the KRAB and MeCP2 inhibition domains from dCas9-KRAB-MeCP2 (Addgene 110821) by inverse PCR. .. We circularized the PCR product using KLD Enzyme Mix (NEB) to generate a new backbone, and we inserted the puromycin resistance cassette which was amplified from Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2. Article Snippet: .. To generate pXR003_puroR (Addgene #219819), we amplified the puromycin resistance cassette from Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2. Article Snippet: To generate the CMV-dCas9_puroR vector (Addgene #219825), we first removed the KRAB and MeCP2 inhibition domains from dCas9-KRAB-MeCP2 (Addgene 110821) by inverse PCR. .. We circularized the PCR product using KLD Enzyme Mix (NEB) to generate a new backbone, and we inserted the puromycin resistance cassette which was amplified from Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2. Article Snippet: To generate miniTurbo-dCasRx (Addgene #219821), we omitted the BASU biotin ligase from CARPID BASU-dCasRx (Addgene 153209) by inverse PCR, and we amplified and inserted the miniTurbo biotin ligase from pcDESTpcDNA5-miniTurboID-3xFLAG-N-term (from Dr. Gingras Lab) into the modified dCasRx vector using the NEBuilder HiFi DNA Assembly Master Mix (NEB). .. To generate pXR004_puroR (Addgene #219820), we added the puromycin resistance cassette from Clone Assay:Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2 Article Snippet: .. We designed nine sgRNAs , using Benchling, ensuring an on- and off-target score greater than 70%, and BbsI restriction cloned them into Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2. Article Snippet: .. We designed nine sgRNAs113,115 using Benchling, ensuring an on- and off-target score greater than 70%, and BbsI restriction cloned them into Transfection:Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2. Article Snippet: .. 24 hours later, we transfected each plate with 48 μg dCas9-miniTurbo (Addgene #219822), 4.8 μg sgRNA even or odd plasmid pool in Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2 Article Snippet: .. 24 h later, we transfected each plate with 48 μg dCas9-miniTurbo (Addgene #219822), 4.8 μg sgRNA even or odd plasmid pool in Plasmid Preparation:Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2. Article Snippet: .. 24 hours later, we transfected each plate with 48 μg dCas9-miniTurbo (Addgene #219822), 4.8 μg sgRNA even or odd plasmid pool in Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2 Article Snippet: .. 24 h later, we transfected each plate with 48 μg dCas9-miniTurbo (Addgene #219822), 4.8 μg sgRNA even or odd plasmid pool in Control:Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2. Article Snippet: .. 24 hours later, we transfected each plate with 48 μg dCas9-miniTurbo (Addgene #219822), 4.8 μg sgRNA even or odd plasmid pool in Article Title: LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2 Article Snippet: .. 24 h later, we transfected each plate with 48 μg dCas9-miniTurbo (Addgene #219822), 4.8 μg sgRNA even or odd plasmid pool in |